Research Program PRRS Analysis Post Doctoral Fellowships PigGen Canada
Research Program Development
Research Projects PCVAD Risk-Assessment Matrix Pig Gen - Gilt Acclimation 2 Pig Gen - Gilt Acclimation 1 Brachyspira hyodysenteriae detection Single-Shot PRRSV Vaccine Control of PFTS Control of haemorrhagic colitis Microarray-based diagnostic tool Nano-volume quantitative PCR Platform PRRS Sequence Analysis PFTS in Pigs in Ontario Tilmicosin
Research Projects
Brachyspira hyodysenteriae detection
Detection of Brachyspira hyodysenteriae from asymptomatic carrier pigs: Comparison of detection methods between individual and pooled fecal samples by direct real time PCR on fresh samples and on samples pre-treated in a selective culture medium.
Project Objectives and Deliverables:
- Compare the detection level for Brachyspira hyodysenteriae using a Real Time Polymerase Chain Reaction directly on feces and indirectly on the Brachyspira enrichment broth inoculated with these feces, in samples collected from asymptomatic pigs or feeder pigs with diarrhea.
- Compare the detection level for Brachyspira hyodysenteriae using a Real Time Polymerase Chain Reaction on pooled versus individual pig fecal samples, with and without prior enrichment procedures.
- Make recommendations for the optimal sampling and PCR testing protocols for detection of Brachyspira hyodysenteriae in large populations of clinically normal pigs when this is needed.
Current Status: As of July 13, 2011, two positive samples have been detected in feeder pig feces submitted from a herd with a suspected outbreak of swine dysentery. Direct testing of feeder pig feces collected from five other herds is currently underway. When this is completed, testing of pooled samples spiked with a known quantity of the positive feces will begin to evaluate the sensitivity of the test in pooled samples with at least one positive sample.
Principle Investigator: Dr. Brendan O’Conner, Prairie Diagnostic Services, Saskatoon